Uji Efektivitas Ekstrak Daun Binahong (Anredera cordifolia (TEN) Steenis) terhadap Jumlah Fibroblas dan Ketebalan Kolagen pada Luka Infeksi Tikus Wistar
Abstract
(Anredera cordifolia) on the healing process of incision wounds infected by Staphylococcus aureus. The ethanol extract of binahong leaves is made in the form of ointments to facilitate the application and release of the drug
into the skin layer. The study used 24 male Wistar rats which were divided into 4 treatment groups namely the group without treatment, the group with fusidic acid ointment, the group with 20% extract of binahong leaf ethanol extract and the group with 40% extract of binahong leaf ethanol. Infected mice were treated and then observed the number of fibroblasts and collagen thickness on day 3 and day 7. Data was analyzed using One Way Anova followed by Duncan Test. The results showed that the ethanol extract of binahong leaves 40% could increase the number of fibroblasts on the 3rd day (43.33 ± 8.08) and the 7th day (61.67 ± 7.02), the ethanol extract of the binahong leaf 20% on 3rd day (31.67 ± 5.50) and 7th day (57.00 ± 7.55). The ethanol extract of binahong leaves 40% increased the thickness of collagen on the 3rd day (10.37 ± 2.69) and the 7th day (16.55 ± 0.32), the ethanol extract of the binahong leaf 20% on the 3rd day ( 8.87 ± 0.71) and 7th day (12.09 ± 1.26). Based on the results of the study, it can be concluded that the ethanol extract of binahong leaves 40% can accelerate the healing process of wound infections by increasing the number of fibroblasts and collagen thickness better than the administration of ethanol extracts of binahong leaves 20%.
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Barbul, A. 2005, Wound Healing in Brunicardi, C. F., Andersen, D. K, Billiar, R. T., Dunn, L. D., Hunter, G. J., Matthews, B. J., Pollock, R. E., Schwartz’s principles of surgery, 8th ed., McGraw-Hill Companies, New York, pp 46-223.
Fitra, N. 2008, Pola kuman aerob dan sensitifitas pada gan-
gren diabetic, Tesis, Sarjana Kedokteran, Universitas Sumatera Utara, Medan.
Gurib-Fakim, A. 2008, Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med. 27(1): 1-93.
Ike, S. M. R. 2001, Pengelolaan Nyeri Pasca Bedah I, National Congress Indonesian Pain Society, Jakarta, pp 58-62.
Jawetz, E., Melnick, J. L. dan Adelberg E. A. 2005, Mikrobiologi Kedokteran, Edisi 23, EGC, Jakarta.
Khan, I., Kumar, N., Pant, I., Narra, S., Kondaiah, P. 2012, Activation of TGF-β pathway by areca nut constituents: a possible cause of oral submucous fibrosis, Plos One, 7(12): 1-12.
Kimura, Y., Sumiyoshi, M., Kawahira, K., Sakanaka, M. 2006, Effects of ginseng saponins isolated from red ginseng roots on burn wound healing in mice, Br. J. Pharmacol, 148(6): 860-870.
Kumar, V., Ramzi, S. C. dan Stanley L. R. 2007, Buku Ajar Patologi Robbins, Edisi 7, EGC, Jakarta.
Morison, M. J. 2004, Manajemen Luka, Penerbit Buku Kedokteran EGC, Jakarta.
Paju, N., Paulina V. Y., Yamleon dan Kojong, N. 2013, Uji
efektivitas salep ekstrak daun binahong (Anredera cordifolia (Ten) Steenis) pada kelinci (Oryctolagus cuniculus) yang terinfeksi bakteri Staphylococcus aureus, Jurnal Ilmiah Farmasi, 2(1): 52-63.
Paskartini, T. G. 2017, ‘Parameter standarisasi tanaman segar, simplisia dan ekstrak etanol daun binahong (Anredera cordifolia) dari tiga daerah berbeda’, Skripsi, Sarjana Farmasi, Universitas Katholik Widya Mandala, Surabaya.
Robbin, 2007, Buku Ajar Patologi, Volume 1, EGC, Jakarta.
Sumartiningsih, S. 2012, The benefit of topically administered binahong for treatment of sport injury (hematoma). Research and Application on Traditional Complementary and Alternative Medicine in Health Care (TCAM), 22(1): 28-31.
Susetya, D. 2010, Khasiat daun Binahong, Pustaka Baru Press, Yokyakarta.
Voigt, R., 1995, Buku Pelajaran Teknologi Farmasi, Edisi 5, Diterjemahkan dari Bahasa Inggris oleh Soendani N. S., Gajah Mada University Press, Yogyakarta. pp 564, 568, 577-578.
DOI: https://doi.org/10.33508/jfst.v6i2.2236
