The Effect Of 1,25-dihydroxyvitamin D To The Phagositosis Activity Of Children Macrophage In Tuberculosis Infection

Rosi Esa Gustina

Abstract


Introduction: Children infected with TB do not always experience TB pain, depending on the number of bacteria that enter and the amount of endurance the child has. 1,25-dihydroxyvitamin D (1,25 (OH) 2D) is a function modulator for macrophages. Objective: This study aims to determine the effect of giving 1,25 (OH) 2D on the phagocytic activity of macrophages in children infected with TB. Methods: This study was an experimental Post Test Only Control Group Design study of 27 samples of children infected with TB. The 1,25 (OH) 2D concentration used was 10-8M and the concentration of 10-7M. Examination of phagocytic activity is carried out using latex beads. Results: One Way ANOVA analysis showed a significant difference in the percentage of macrophages that phagocyte latex and the average number of latex phagocytes by each macrophage between the control group and the treatment group (p <0.05). In the Post Hoc Bonferroni analysis, the mean percentage of macrophages that phagocyted latex was significant in group K with P2 (p = 0.01) and group P1 with P2 (p = 0.03), while the mean percentage of macrophages that phagocyted latex was not significant in group K with group P1 (p = 0.13). The mean number of latex particles phagocyted by each macrophage was significantly found in group K with P1 (p = 0.01) and group K with P2 (p = 0.01), while the mean number of latex particles phagocytized by each macrophage was not significantly found in group P1 with group P2 (p = 0.91).

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Keywords


1,25 dihydoxyvitamin D, Phagocytosis, Tuberculosis

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References


World Health Organization. Tuberculosis-Epidemiology, Tubercu-losis; Pulmonary-Prevention and control, Tuberculosis-Economics, Tuberculosis; Multidrug-Resistant, Annual Reports. Global Tuberculosis Report 2013: WHO Library Cataloguing in Publication Data; 2013.

Kemenkes RI. Petunjuk Teknis Manajemen TB Anak. Jakarta: Direktorat Jenderal Pengendalian Penyakit dan Penyehatan Lingkungan Kementrian Kesehatan RI; 2013.

Crofton J, Horne N, Miller F. Tuberkulosis Klinis. Jakarta:Widya Medika; 2002.

Aditama TY. Petunjuk Teknis Tata Laksana Klinis Ko-Infeksi TB-HIV. Jakarta: Kementrian Kesehatan RI; 2012.

Nielsen NO, Skifte T, Anderson M, Wohlfahrt J, Soborg B, Koch A, Melbye M, Ladefoged K. Both High and Low Serum Vitamin D Concentration are Associated with Tuberculosis: A Case-Control Study in Greenland. British J of Nutrition. 2010; 104:1481-87.

Hansdottir S and Monick MM. Vitamin D Effect on Lung Immunity and Respiratory Diseases. Vitam Horm. 2011; 86: 217-37.

Fabri M, Stenger S, Shin DM, Yuk JM, Liu PT, Realegeno S, et al Vitamin D is Required for IFN- γ-Mediated Antimicrobial Activity of Human Macrophages. Science Translational Medicine. 2011; 3 (104): 102-04.

Liu PT, Stenger S, Li H, Wenzel L, Tan BH, Krutzik SR, et al. Toll Like Receptor Triggering of Vitamin D Mediated Human Antimicrobial Response. Science. 2006; 311 (5768): 1770-73.

Sook Lee J, Su Yang C, Shin DM, Yuk JM, Son JW, Jo EK. Nitric Oxide Synthesis is Modulated by 1,25-Dihydroxyvitamin D3 and Interferon-γ in Human Macrophages after Mycobacterial Infection. Immune. 2009; 9 (5) :192-202.

Campbell GR and Spector SA. Vitamin D Inhibits Human Immunodeficiency Virus Type 1 and Mycobacterium tuberculosis Infection in Macrophages through the Induction of Autophagy. PLoS Pathog. 2012; 8(5): 26-89.

Fabri, M. and Modlin, R. L. A Vitamin for Autophagy. Cell Host and Microbe. 2009; 6 (3): 201–03.

Yuk JM, Shin DM, Lee HM, Yang CS, Jin HS, Kim KK, et al. Vitamin D3 Induces Autophagy in Human Monocytes/Macrophages Via Cathe-licidin. Cell Host and Microbe. 2009; 6 (3): 231–43.

Tjahajati, Ida. Vaksinasi BCG Meningkatkan Aktivitas Fagositosis dan Sekresi Reaktif Oxigen Intermediate (ROI) pada Makrofag Peritonium Kucing yang Diinfeksi dengan Mycobacterium tuberculosis. J Kedokteran Brawijaya. 2005; 21 (2): 1-8.

Marusin S dan Chairul. Efek Ekstrak Air dan Alkohol Siwak (Salvadora persica L.) terhadap Penngkatan Aktivitas dan Kapasitas Fagositosis Sel Makrofag. Media Litbang Kesehatan. 2012; 22 (1).

Di Rosa M, Malaguarnera M, Nicoletti F and Malaguarnera L. Vitamin D3: A Helpful Immuno Modulator. Immunology. 2011;134: 123–39.

Arora VK and Jaiswal AK. Vitamin D Receptor Polymorphism and Active Tuberculosis. Indian J Tuberc. 2013; 60 (4): 199-201.

Chandra G, Selvaraj P, Jawahar MS, Banurekha V, Narayanan PR. Effect of Vitamin D3 on Phagocytic Potential of Macrophages with Live Mycobacterium tuberculosis and Lymphoprolife-rative Response in Pulmonary Tuberculosis. J Clin Immunol. 2004; 24(3): 249-57.

Kobayashi H, Asano K, Kanai K and Suzaki H. Suppressive Activity of Vitamin D3 on Matrix Metalloproteinase Production From Cholesteatoma Keratinocytes In Vitro. Mediators Inflamm. 2005; 4: 210–15.

Bacchetta J, Sea JL, Chun RF, Lisse TS, Wesseling-Perry K, Gales B, et al. FGF23 Inhibits Extra-Renal Synthesis of 1,25-dihydroxyvitamin D in Human Monocytes. J Bone Miner Res. 2013; 28 (1 ): 46–55.

Agmon-Levin N, Blank M, Zandman-Goddard G, Orbach H, Meroni PL, Tincani A, et al. Vitamin D: An Instrumental Factor in The Anti-phospholipid Syndrome by Inhibition of Tissue Factor Expression. Ann Rheum Dis. 2011; 70: 145–50.

Kang SN, Kim SH, Chung SW, Lee MH, Kim HJ, Kim TS. Enhanment 0f 1α, 25-Dihydroxyvitamun D3 Induced of Human Leukaemia HL-60 Cells into Monocytes by Parthenolide Via Inhibition of NF-kB Activity. British J of Pharm. 2002; 135: 1235-44.

Uitterlinden AG, Fang Y, Van Meurs, Van Leeuwen H, Pols HA. Vitamin D Receptor Gene Polymorphisms in Relation to Vitamin D Related Disease States. Journal of Steroid. Biochemistry & Molecular Biology. 2004: 187–193.

Stoffels K, Overbergh L, Giulietti A, Verlinden L, Bouillon R and Mathieu C. Immune Regulation of 25-Hydroxyvitamin D3 1α-Hydroxylase in Human Monocytes. JBMR. 2006; 21 (1): 37-47.

Nelson CD, Reinhardt TA, Beitz DC and Lippolis JD. In Vivo Activation of the Intracrine Vitamin D Pathway in Innate Immune Cells and Mammary Tissue during a Bacterial Infection. PLoS ONE. 2010; 5 (11): 1-7.

Bikle DS. Vitamin D: Production, Metabolism, Mechanism of Action: Endotext. 2009.

Champe PC, Richard AH dan Deniese RF. Biokimia Ulasan Bergambar. Jakarta: EGC; 2011.




DOI: https://doi.org/10.33508/jwm.v6i1.2502