Efek kombinasi metformin-oksigen hiperbarik terhadap jumlah fibroblas dan ketebalan kolagen dalam penyembuhan luka pada tikus putih hiperglikemia

Wahyu Dewi Tamayanti, Bernadette Dian Novita Dewi, Imelda Theodora

Abstract


Background : Metformin is used in patients with type 2 DM who had a chronic hyperglycemia caused by insulin resistance. DM patients also suffered from hypoxia condtion caused by a lacking oxygen in the tissues.
Aim : This study was aimed to identify the effect of combination therapy of metformin and hyperbaric oxygen to increase the number of fibroblasts and collagen thickness in the Pseudomonas aeruginosa-infected tissue in male Wistar rats with hyperglycemia.
Methods : This was an experimental study using post test only control design. Male Wistar rats (Rattus norvegicus) were divided into five groups: KN - was the group of normal Wistar rats; KDM - was the group with alloxan induction; KI - was the group with alloxan induction and infected with Pseudomonas aeruginosa without treatment; KU1 - was the group induced with alloxan, infected with Pseudomonas aeruginosa, and treated with metformin; and KU2 – was the group that induced with alloxan, infected with Pseudomonas aeruginosa and treated with combination therapy of metformin and hyperbaric oxygen. This study used alloxan 8% (w/v) that was injected intra muscular to induce hyperglycemic condition. Anaerobic bacteria Pseudomonas aeruginosa was swapped in the injured area. The treatment dose of metformin was 45mg/kgBB and hyperbaric oxygen therapy at 2.4 ATA given 3 times 30 minutes respectively with 5 minutes interval for 5 consecutive days. Skin tissue was taken on the termination and HE stained to analys the number of fibroblast and sized the collagen. Data obtained was statistically analysed by one way anova with a significant level of 5%.
Result : The result confirmed that combination therapy of metformin and hyperbaric oxygen increases significantly the number of fibroblasts and collagen thickness in the wound of male hyperglycemic Wistar rats infected with Pseudomonas aeruginosa.
Discussion : Combination therapy of metformin and hyperbaric oxygen enhanced wound healing by enhancing fibroblast and increasing the thickness of collagen.

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