Analysis Of Antibacterial Effect Of Curcuminoid With Mesoporous Silica Nanoparticles On Staphylococcus Epidermidis

Ferdinand Erwin, Bernadette D Novita, Galuh N Prawesti

Abstract


Introduction: The rate of antibiotic resistance in Staphylococcus epidermidis is high. The previous study shows that 79% of Staphylococcus epidermidis bacteria isolated were resistant to Methicillin, while 98% were resistant to Penicillin. The active substance Curcuminoid in Turmeric is known to have an antibacterial effect. Nowadays, nanoparticle-based medicine has been developed which can increase bioavailability and solubility in the water, as well as the cellular uptake. Aim: The study aimed to analyze the inhibitory and killing effect of Curcuminoid from Turmeric (Curcuma longa L.) with mesoporous silica nanoparticles against Staphylococcus epidermidis Methods: This research performs a microdilution test of Curcuminoid with mesoporous silica nanoparticles at concentration 2000 – 32000 μg/mL against Staphylococcus epidermidis on the microplate. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained from its optical density value after evaluating its turbidity on the spectrophotometer. Results: In this research found the MIC of Curcuminoid with mesoporous silica nanoparticles against Staphylococcus epidermidis was at the concentration of 32000 μg/mL. Conclusion: Curcuminoid from turmeric (Curcuma longa L.) with mesoporous silica nanoparticles has an inhibitory effect against Staphylococcus epidermidis

Save to Mendeley


Full Text:

PDF

References


Carroll KC, Butel J, Mietzner TA. Jawetz, Melnick, & Adelberg’s Medical Microbiology. 27th ed. Carroll KC, Hobden JA, Miller S, Morse SA, Mietzner TA, editors. New york: McGraw-Hill Education; 2015. 864 p.

D. Fey P. Staphylococcus Epidermidis Methods and Protocols. Vol. 1106, Methods in Molecular Biology. 2014.

Büttner H, Mack D, Rohde H. Structural basis of Staphylococcus epidermidis biofilm formation: mechanisms and molecular interactions. Front Cell Infect Microbiol [Internet]. 2015;5(February):1–15. Available

from:http://journal.frontiersin.org/Article/10.3389/fcimb.2015.00014/abstrac

Crossley KB, Jefferson KK, Archer GL, Fowler VG. Staphylococci in

Human Disease. 2nd ed. Vol. 53, Journal of Chemical Information and Modeling. Blackwell Publishing; 2009. 310-332 p.

Kleinschmidt S, Huygens F, Faoagali J, Rathnayake IU, Hafner LM.

Staphylococcus epidermidis as a cause of bacteremia. Future Microbiol

[Internet]. 2015;10(11):1859–79. Available from: http://www.futuremedicine.com/doi/10.2217/fmb.15.98

Mendes RE, Deshpande LM, Costello AJ, Farrell DJ. Molecular Epidemiology of Staphylococcus epidermidis clinical isolates from U.S.

hospitals. Antimicrob Agents Chemother. 2012;56(9):4656–61.

Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci.

Clin Microbiol Rev. 2014;27(4):870–926.

Otto M. Molecular basis of Staphylococcus epidermidis infections. Semin Immunopathol 2012;34(2):201–14.

Gordon RJ, Miragaia M, Weinberg AD, Lee CJ, Rolo J, Giacalone JC, et al. Staphylococcus epidermidis colonization is highly clonal across US cardiac centers. J Infect Dis.2012;205(9):1391–8.

Perdagangan K. Obat Herbal Tradisional. War Ekspor. 2014;(September 2014):1–20.

Zorofchian Moghadamtousi S, Abdul Kadir H, Hassandarvish P, Tajik H,

Abubakar S, Zandi K. A review on antibacterial, antiviral, and antifungal

activity of curcumin. Biomed Res Int. 2014;2014.

Araújo CC, Leon LL. Biological activities of Curcuma longa L. Mem

Inst Oswaldo Cruz [Internet]. 2001 Jul;96(5):723–8. Available from:

http://www.ncbi.nlm.nih.gov/pubmed/11500779

Simanjuntak P. Studi Kimia dan Farmakologi Tanaman Kunyit (Curcuma longa L) Sebagai Tumbuhan Obat Serbaguna. Lemb Ilmu Pengetah Indones [Internet]. 2012;17(2):103. Available from: http://download.portalgaruda.org/article.php?article=293764&val=6157&title=STUDI KIMIA DAN FARMAKOLOGI TANAMAN KUNYIT (Curcuma longa L) SEBAGAI TUMBUHAN OBAT SERBAGUNA

Bhawana, Basniwal RK, Buttar HS, Jain VK, Jain N. Curcumin

Nanoparticles : Preparation , Characterization , and Antimicrobial

Study. J Agric Food Chem. 2011;59:2056–61.

Anwar E, Iswandana R, Mun’im A. Uji Penetrasi Secara In Vitro dan Uji

Stabilitas Fisik Sediaan Krim, Salep, dan Gel, Yang Mengandung Kurkumin dari Kunyit (Curcuma longa L.). J Bahan Alam Indones. 2011;7(7): 370–4.

Bone K, Mills S. Herbal therapeutic systems. In: Principles and Practice of Phytotherapy [Internet]. 2nd ed. London: Elsevier; 2013. p. 3–16.

Available from: http://linkinghub.elsevier.com/retrieve/pii/B9780443069925000013

Kali A, Bhuvaneshwar D, Charles P V., Seetha K. Antibacterial synergy of

curcumin with antibiotics against biofilm producing clinical bacterial

isolates. J Basic Clin Pharm [Internet]. 2016;7(3):93. Available from: http://www.jbclinpharm.org/text.asp?2016/7/3/93/183265

Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. Bioavailability of Curcumin : Problems and Promises. Mol Pharm. 2007;4(6):807–18.

Bitar A, Ahmad NM, Fessi H, Elaissari A. Silica-based nanoparticles

for biomedical applications. Drug Discov Today [Internet]. 2012;17(19–

:1147–54. Available from: http://dx.doi.org/10.1016/j.drudis.201

06.014

Tang L, Cheng J. Nonporous SilicaNanoparticles for Nanomedicine

Application. Nano Today.

;8(3):290–312. 21. Liberman A, Mendez N, Trogler WC, Kummel AC. Synthesis and surface functionalization of silica nanoparticles for nanomedicine. Surf Sci Rep. 2014;69(2–3):132–58.

Aneja KR. Experiments in Microbiology and Plant Pathology

and Biotechnology. 4th ed. New Delhi: New Age International Pvt.

Ltd.; 2003. 216 p.

Indriana. Uji Banding Efektivitas Ekstrak Rimpang Temu Kunci

(Kaemferia pandurata Roxb) 10% dengan Ketokonazol 2% Secara In

Vitro Terhadap Pertumbuhan Candida albicans Pada Kandidiasis vaginalis.

Artikel Ilmiah Hasil Penelitian Mahasiswa. UNIVERSITAS DIPONEGORO; 2006.

Gaikwad A, Bodhankar M, Ittadwar A, Waikar S. Antibacterial activity of

isoflavone extracted from Curcuma longa linn. Zingeberaceae. ISOI J

Microbiol Biotechnol Food Sci.2014;1(1):6–9.

Zhou X, Li Y. Atlas of Oral Microbiology: From Healty Microflora to Disease. London: Academic Press; 2015. 68 p. 26. Niamsa N, Sittiwet C. Antimicrobial Activity of Curcuma longa Aqueous Extract. J Pharmacol Toxicol [Internet]. 2009 Apr 1;4(4):173–7. Available from: http://www.scialert.net/abstract/?doi=jpt.2009.173.177

Kharat M, Du Z, Zhang G, McClements DJ. Physical and Chemical Stability of Curcumin in Aqueous Solutions and Emulsions: Impact of pH, Temperature, and Molecular Environment. J Agric Food Chem [Internet]. 2017 Mar 16;65(8):1525–32. Available from: http://pubs.acs.org/doi/10.1021/acs.jafc.6b04815




DOI: https://doi.org/10.33508/jwmj.v1i2.2019

Refbacks

  • There are currently no refbacks.