ANTIBACTERIAL EFFECT OF EUGENIA POLYANTHA WIGHT WITH CORYNEBACTERIUM DIPHTHERIAE NON-TOXIGENIC

Ivita T Murbarani, Endang Isbandiati, Chrisdina Puspitasari

Abstract


Introduction: Corynebacterium diphtheriae is a bacteria that causes diphtheria. In recent
years, the bacterium is resistant to antibiotics. Eugenia polyantha wight might be a suitable
antibiotic alternative as it has antibacterial effect against Staphylococcus aureus. The content
of the antibacterial compound in Eugenia polyantha wight at specific concentrations is
expected to be able to kill the Corynebacterium diphtheriae.
Purpose: This study aimed to examine the antibacterial effect of Eugenia polyantha wight to
Corynebacterium diphtheriae non-toxic.
Method: Bay leaf extract (Eugenia polyantha wight) made with maceration method. This
research is an experimental study conducted in vitro using a microdilution test on 96 well
plates then count value with the streaking method to find out MBC.
Results: bactericidal power was quite effective at the concentration of 40%, while at the concentration
of 80%-100% has very strong bactericidal power that could destroy the growth of bacteria 99.9%. The
higher the concentration of bay leaves extract, the higher the bactericidal power of bacteria
Conclusion: Eugenia polyantha wight has an antibacterial effect against Corynebacterium
diphtheriae non-toxic.


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Keywords


MBC; bay leaf; Eugenia polyantha wight; Corynebacterium diphtheriae non toxic.

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References


Baron S. Medical Microbiology. 4th edition. University of Texas

Medical Branch at Galveston. 1996.

Dinas kesehatan provinsi jawa timur. No Title. Penanggulangan

KLB Difteri di Provinsi Jawa Timur. Surabaya: IDAI Cabang Jawa Timur; 2018. 9–11 p.

Pereira GA, Pimenta FP, Dos Santos FRW, Damasco PV, Hirata R,

Mattos-Guaraldi AL. Antimicrobial resistance among Brazilian

Corynebacterium diphtheriae strains. Mem Inst Oswaldo Cruz. 2008;103(5):507–10.

Ismail A, Ahmad WANW. Syzygium polyanthum (Wight) Walp: A

Potential Phytomedicine. Pharmacogn J. 2019;11(2):429–38.

Sumono A, SD AW. The use of bay leaf (Eugenia polyantha Wight) in

dentistry. Dent J (Majalah Kedokt Gigi). 2016;41(3):147.

Harismah K, Chusniatun. Pemanfaatan Daun Salam (Eugenia

polyantha) Sebagai Obat Herbal dan Rempah Penyedap Makanan. War

LPM. 2016;

Apriani D, Amaliawati N, Kurniati E. Efektivitas Berbagai Konsentrasi

Infusa Daun Salam (Eugenia polyantha Wight) terhadap Daya

Antibakteri Staphylococcus aureus Secara In Vitro. Teknol Lab. 2014;

Edition E. Brock Biology of Microorganisms. Cell. 2006;

Revathi G, Talwar V. A biochemical test based on pyrazinamidase activity for rapid differentiation ofcorynebacteria. Indian J Clin

Biochem. 1995;

Putranto RH, Sariadji K, Sunarno, Roselinda. Corynebacterium

diphtheriae: Diagnosis Laboratorium Bakteriologi. 2014. p. 67.

Colman G, Weaver E, Efstratiou A. Screening tests for pathogenic

corynebacteria. J Clin Pathol. 1992;

Smith LD. Texbook of Microbiology. William Burrows. Saunders, Philadelphia, Pa., ed.17, 1959. xxxiii + 954 pp. Illus. $14. Science (80- ). 1960;

Lim D. Microbiology. New York: McGraw-Hill; 2006.

Sariadji K, Sunarno S, Khariri K, Puspandari N, Muna F, Rukminiati

Y. Selektivitas Medium Cystine Tellurite Blood Agar (CTBA) terhadap Beberapa Isolat Bakteri. J Kefarmasian Indones. 2017;5(1):19– 24.

Dwi Krihariyani, Evy Diah Woelansari EK. Pola Pertumbuhan Staphylococcus aureus Pada Media Agar Darah Manusia Golongan O, AB, dan Darah Domba Sebagai Kontrol. Ilmu dan Teknol Kesehat. 2016;3,nomor 2.

Taylor PC, Schoenknecht FD, Sherris JC, Linner EC. Determination of minimum bactericidal concentrations of oxacillin for Staphylococcus aureus: Influence and significance of technical factors. Antimicrob Agents Chemother. 1983;

Sri Agung FK, Ramdhani D, Mustarichie R. Comparative study on

activities of anti bacillary dysentry Shigella dysenteriae of Syzygium

polyanthum and Dracaena angustifolia leaves ethanol extracts.

Asian J Pharm Clin Res. 2017;

Asmira Abd Rahim EN, Ismail A, Omar MN, Rahmat UN, Nizam

Wan Ahmad WA. GC-MS analysis of phytochemical compounds

in syzygium polyanthum leaves extracted using ultrasound-assisted method. Pharmacogn J. 2018;

Lee W, Woo ER, Lee DG. Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa. Free Radic Res. 2016




DOI: https://doi.org/10.33508/jwmj.v3i2.3182

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