PENGARUH KONSENTRASI NATRIUM ALGINAT SEBAGAI PENJERAT SEL Lactobacillus acidophilus FNCC 0051 DAN LAMA PENYIMPANAN TERHADAP JUMLAH SEL YANG TERLEPAS DAN KARAKTER CARRIER

Abstract

The aim of this study is to know the effect of sodium alginate concentration in Lactobacillus acidophilus FNCC 0051 entrapment, refrigeration storage (±5oC) period and interaction of both factors on the number of released cells and carrier’s character (UHT milk). The experimental design used was factorial RBD (Randomized Block Design) design with two factors, sodium alginate concentration (1%, 1,5%, 2%) and storage period (0,10, 20 days). The parameters observed are the number of released cells (Total Plate Count / TPC), pH and total lactic acid of the carrier (UHT milk). The obtained data are statistically analyzed by ANOVA (Analysis of Variance) at α=5% and continued with Duncan’s Real Difference Distance test (Duncan's Multiple Range Test) to determine which level of the treatment that gives a significant differences. Sodium alginate concentration and storage period had significant effect on the number of released cells and carrier’s character (pH and total lactic acid of UHT milk) and there is interaction between both factors. The lower level of sodium alginate concentration increased the number of released cells, decreased pH value and increased total lactic acid. During 20 days storage, the amount of released cells and acidity of carrier increased than 0 and 10 days. Interaction between both factors which give the less number of released cells (3,7948 log cfu/ml) and carrier’s character changes (pH = 6,4767 and total lactic acid= 0,0741 %) is combination of 2% sodium alginate concentration in 0 days storage.

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